RT-PCR SERVICES

  • RT-PCR service

Genechron offers a complete set of semi-quantitative real - time PCR (RT-PCR) assays for gene expression analysis

Real-time PCR (RT-PCR) assays

Genechron offers a complete set of semi - quantitative real - time PCR (RT-PCR) assays for gene expression analysis. Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays can detect quantify every human, mouse rat transcripts RefSeq database. Mouse rat assays designed strain - neutral. specialists can design custom gene expression assays all sequences variants from other organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting.Genechron offers complete set semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis. Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays detect quantify every human, mouse rat transcripts RefSeq database. Mouse rat assays designed strain - neutral. specialists design custom gene expression assays sequences variants organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting.Genechron offers complete set semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays detect quantify every human, mouse rat transcripts RefSeq database Mouse rat assays designed strain - neutral specialists can design custom gene expression assays sequences variants organisms. Genechron service includes isolation RNA sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting. Genechron offers complete set of semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assaysdetect quantify every human, mouse rat transcripts RefSeq database Mouse rat assays designed strain - neutral specialists design custom gene expression assays sequences variants from other organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting Genechron offers a complete set of semi - quantitative real - time PCR (RT-PCR) assays for gene expression analysis. Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays can detect quantify every human, mouse rat transcripts RefSeq database. Mouse rat assays designed strain - neutral. specialists can design custom gene expression assays all sequences variants from other organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting.Genechron offers complete set semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis. Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays detect quantify every human, mouse rat transcripts RefSeq database. Mouse rat assays designed strain - neutral. specialists design custom gene expression assays sequences variants organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting.Genechron offers complete set semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assays detect quantify every human, mouse rat transcripts RefSeq database Mouse rat assays designed strain - neutral specialists can design custom gene expression assays sequences variants organisms. Genechron service includes isolation RNA sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting. Genechron offers complete set of semi - quantitative real - time PCR (RT-PCR) assays gene expression analysis Our assays consist both TaqMan Sybr-Green based RT-PCR garantee high specificity, high sensitivity dynamic range least 7 log. Assaysdetect quantify every human, mouse rat transcripts RefSeq database Mouse rat assays designed strain - neutral specialists design custom gene expression assays sequences variants from other organisms. Genechron service includes isolation RNA from your sample (demand), cDNA convertion, design custom assay (design oprimers/probes PCR optimization), RT-PCR, experimental execution, data analysis reporting.

Service

Genechron offers a complete set of semi-quantitative real - time PCR (RT-PCR) assays for gene expression analysis. Our assays consist in both TaqMan and Sybr-Green based RT-PCR and garantee high specificity, high sensitivity, and a dynamic range of at least 7 log. Assays can detect and quantify every human, mouse and rat transcripts in the RefSeq database. Mouse and rat assays are designed to be strain - neutral. Our specialists can design custom gene expression assays for all sequences and variants from other organisms.


Offers

Genechron service includes isolation of RNA from your sample (on demand), cDNA convertion, design of the custom assay (design of primers/probes and PCR optimization), RT-PCR, experimental execution, data analysis and reporting. 

Description of Service

    Sample preparation:
  • From biological samples: RNA isolation from samples (cells or frozen tissue, serum, plasma, urine, saliva) and first strand cDNA synthesis
  • From RNA: first strand cDNA synthesis¬†
    Experimental Design and Execution:
  • Semi-quantitative Assay design
  • RT-PCR carried out in triplicate on 384 well plates
  • Amplification of the target sequence(s), housekeeping genes and no-template control
    Data Analysis:
  • Analysis of target expression levels relative to housekeeping genes
  • Report of results and assay performance